Lab Publications

Active Site Aromatic Residues Play a Dual Role in the Substrate Interaction and Protein Structure in Functional Dimers of CYP121A1 of Mycobacterium tuberculosis

Christopher S. Campomizzi, Amit Kumar, Patil Pranita Uttamrao, Jack J. Stallone, George E. Chanatios, Thenmalarchelvi Rathinavelan, and D. Fernando Estrada

2023, ACS Infectious Diseases

Collaborative publication with Simons lab:

Synthesis, biological evaluation and computational studies of pyrazole derivatives as Mycobacterium tuberculosis CYP121A1 inhibitors

Lama A. Alshabini, Amit Kumar, Sam J. Willcocks, Gayathri Srithiran, Sanjib Bhakta, D. Fernando Estrada, and Claire simons

2022, RSC Medicinal Chemistry

Characterization of a cleavable fusion of human CYP24A1 with Adrenodoxin reveals the variable role of hydrophobics in redox partner binding

Natalie Jay, Sean R. Duffy, D. Fernando Estrada

2022, Biochemistry

19F-NMR reveals substrate specificity of CYP121A1 in Mycobacterium tuberculosis

Christopher S. Campomizzi, George E. Ghanatios, D. Fernando Estrada

2021, Journal of Biological Chemistry

Surface hydrophobics mediate functional dimerization of CYP121A1 of Mycobacterium tuberculosis

Amit Kumar, Christopher S. Campomizzi, Natalie Jay, Shaun Ferguson, Emelie-Jo Scheffler, James Lioi, Chengjian Tu, Jun Qu, Claire Simons, D. Fernando Estrada

2021, Scientific Reports

Evidence of Allosteric Coupling between Substrate Binding and Adx Recognition in the Vitamin D Carbon-24 Hydroxylase CYP24A1

Amit Kumar, P. Ross Wilderman, Chengjian Tu, Shichen Shen, Jun Qu, D. Fernando Estrada

2020, Biochemistry

Specificity of the redox complex between cytochrome P450 24A1 and adrenodoxin relies on carbon-25 hydroxylation of vitamin-D substrate

Amit Kumar & D. Fernando Estrada

2019, Drug Metabolism and Disposition

The cytochrome P450 24A1 interaction with adrenodoxin relies on multiple recognition sites that vary among species

D. Fernando Estrada

2018, Journal of Biological Chemistry